Table 1 |
||||
|
Primers used for amplification and sequencing of the RBL2 gene. |
||||
|
Exon |
Primer sequence (5'-3') |
Annealing temperature |
Product size (bp) |
|
|
|
||||
|
19-1 |
Forward |
AGGGTGTGCTCAACAAATAC |
50 |
142 |
|
Reverse |
CTGTTGGAGAATTCTGATGG |
|||
|
19-2 |
Forward |
TCTCTAAAGGTGTATAGAAGTG |
50 |
161 |
|
Reverse |
GAAAAGCTCCTAATTTTGTC |
|||
|
20-1 |
Forward |
ATTAGCAGCCTTTGTACTGAC |
55 |
148 |
|
Reverse |
ACTGTTGGCACCTGTGAGG |
|||
|
20-2 |
Forward |
AGCAGTGCTCCTCCCACA |
50 |
174 |
|
Reverse |
AAAGTATGAAATAATCCCTG |
|||
|
21 |
Forward |
CTCTCTCCCTATCCATTTG |
50 |
143 |
|
Reverse |
GGACTGTTGCTGAAGTAATAG |
|||
|
22-1 |
Forward |
CTGAGCTATGTGCATTTGCA |
52 |
148 |
|
Reverse |
TTTGCAGGTGATTCACTTCC |
|||
|
22-2 |
Forward |
AAAGAGAGGAATTCTTTTGG |
54 |
145 |
|
Reverse |
AGAGTTTAATACAAGAGACT |
|||
|
|
||||
|
Kalungi et al. BMC Clinical Pathology 2009 9:6 doi:10.1186/1472-6890-9-6 |
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