Table 2

Summary of specific antisera and immunohistochemistry conditions

Protein

Antibody

Dilution & incubation

Antigen pretreatment

Positive control

Stain


Cyclin D1

P2D11F11 Novocastra

1:50

Microwave

Colon cancer

Nuclear

Cyclin E

13A3 Novocastra

1:80

Microwave

Breast cancer

Nuclear

CDK4

C-22 Santa Cruz Biotechnology

1:300

Microwave

Colon cancer

Nuclear

P53

DO7 DAKO

1:50, 2 h

Microwave

Breast cancer

Nuclear

P21waf

SX118, DAKO

1:50, 2 h

Microwave

Epidermis

Nuclear

Mdm-2

Monoclonal IF2 Oncogene

1:20, 2 h

Microwave

sarcoma

Nuclear

P27

SX53G8, DAKO

1:80, 1 h

None

Breast cancer

Nuclear

P16

6H12 Novocastra Lab

1:40

Microwave

Epidermis

Nuclear

Rb

Rb1(recognizes phosphorylated & non-phosphorylated protein) DAKO

1:80

Microwave

Retinoblastoma

Nuclear

Ki-67

MIB 1, Dako

1:50

Microwave

Breast cancer

Nuclear


Bahnassy et al. BMC Clinical Pathology 2007 7:4   doi:10.1186/1472-6890-7-4

Open Data