Effect of Cholinergic agonists on hepatic expression of cytokine mRNA following IR. RT-PCR analysis of TNF-α, MIP-2, and IL-6 mRNA liver from mice that were pretreated with vehicle (i.e. normal saline), DMPP (1 mg/kg), or nicotine (1 mg/kg), prior to the onset of ischemia (90 min), followed by 3 h of reperfusion (IR). The PCR products were run on 2.5% agarose gel, stained with ethidium bromide, and then visualized by ultraviolet illumination. GAPDH was used as a housekeeping gene. Blots shown are representative of four separate experiments with similar results.
Crockett et al. BMC Clinical Pathology 2006 6:3 doi:10.1186/1472-6890-6-3