Table 2

PCR protocol sensitivities for the detection of HPV-16 in SiHa/C-33A DNA mixtures. SiHa cell DNA (0.1 ng to 100 ng) was mixed with C-33A cell DNA to give final DNA quantities of 2 μg, 1 μg, 0.5 μg, or 0.1 μg. These preparations were tested with up to six different PCR protocols. HPV-16 detection was most successful using by the TDP3 and TDP4 conditions. Data are for protocols tested at 50 PCR cycles. HPV detected, HPV undetected, ND: not done.

Total DNA in PCR: 2 μg

Total DNA in PCR: 1 μg


SiHa DNA

100 ng

25 ng

1 ng

0.5 ng

0.1 ng

100 ng

25 ng

1 ng

0.5 ng

0.1 ng


GP5+/6+

TDP1

TDP2

TDP3

TDP4

ND

ND

ND

ND

ND

MRT

ND

ND

ND

ND

ND

ND

ND

ND

ND

ND


Total DNA in PCR: 0.5 μg

Total DNA in PCR: 0.1 μg


SiHa DNA

100 ng

25 ng

1 ng

0.5 ng

0.1 ng

100 ng

25 ng

1 ng

0.5 ng

0.1 ng


GP5+/6+

TDP1

TDP2

TDP3

TDP4

MRT

ND

ND

ND

ND

ND


Evans et al. BMC Clinical Pathology 2005 5:10   doi:10.1186/1472-6890-5-10

Open Data