Table 2 |
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|
PCR protocol sensitivities for the detection of HPV-16 in SiHa/C-33A DNA mixtures.
SiHa cell DNA (0.1 ng to 100 ng) was mixed with C-33A cell DNA to give final DNA quantities
of 2 μg, 1 μg, 0.5 μg, or 0.1 μg. These preparations were tested with up to six different
PCR protocols. HPV-16 detection was most successful using by the TDP3 and TDP4 conditions.
Data are for protocols tested at 50 PCR cycles. |
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|
Total DNA in PCR: 2 μg |
Total DNA in PCR: 1 μg |
|||||||||
|
|
||||||||||
|
SiHa DNA |
100 ng |
25 ng |
1 ng |
0.5 ng |
0.1 ng |
100 ng |
25 ng |
1 ng |
0.5 ng |
0.1 ng |
|
|
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|
GP5+/6+ |
|
|
|
|
|
|
|
|
|
|
|
TDP1 |
|
|
|
|
|
|
|
|
|
|
|
TDP2 |
|
|
|
|
|
|
|
|
|
|
|
TDP3 |
|
|
|
|
|
|
|
|
|
|
|
TDP4 |
ND |
ND |
ND |
ND |
ND |
|
|
|
|
|
|
MRT |
ND |
ND |
ND |
ND |
ND |
ND |
ND |
ND |
ND |
ND |
|
|
||||||||||
|
Total DNA in PCR: 0.5 μg |
Total DNA in PCR: 0.1 μg |
|||||||||
|
|
||||||||||
|
SiHa DNA |
100 ng |
25 ng |
1 ng |
0.5 ng |
0.1 ng |
100 ng |
25 ng |
1 ng |
0.5 ng |
0.1 ng |
|
|
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|
GP5+/6+ |
|
|
|
|
|
|
|
|
|
|
|
TDP1 |
|
|
|
|
|
|
|
|
|
|
|
TDP2 |
|
|
|
|
|
|
|
|
|
|
|
TDP3 |
|
|
|
|
|
|
|
|
|
|
|
TDP4 |
|
|
|
|
|
|
|
|
|
|
|
MRT |
ND |
ND |
ND |
ND |
ND |
|
|
|
|
|
|
|
||||||||||
|
Evans et al. BMC Clinical Pathology 2005 5:10 doi:10.1186/1472-6890-5-10 |
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HPV detected, 
HPV undetected, ND: not done.