Amplification of HPV from abnormal cervical cell DNA samples by TDP3 and TDP4. Seven samples that tested negative for HPV using standard GP5+/GP6+ cycling conditions were assessed with the TDP3 and TDP4 assays. Both TDP3 and TDP4 supported the detection of HPV in sample 4 when 100 ng DNA was subject to PCR but not when 500 ng DNA was in a reaction. HPV was detected in sample 2 by the TDP3 protocol with 100 ng or 500 ng template DNA and by the TDP4 protocol at 500 ng but not 100 ng of sample. RB: reagent blank.
Evans et al. BMC Clinical Pathology 2005 5:10 doi:10.1186/1472-6890-5-10