Figure 3.

Detection of recombinant HPV plasmid by GP5+/GP6+ PCR. HPV (types 16, 31, 33, 45, 51, 52, and 56) recombinant plasmids, 0, 15ag, 150ag, 1.5fg, 15fg, 150fg, 1.5pg, were diluted in 100 ng C-33A DNA and amplified (50 cycles) using the GP5+/GP6+ protocol. Positive control amplification of HPV-18 from 100 ng HeLa cell DNA is also shown.