Southern blot of TDP3 and MRT GP5+/GP6+ PCR products. SiHa cell DNA, 0, 0.1, 0.5, 1.0, 25, or 100 ng, was diluted into a total of 0.1 μg DNA made up with C-33A DNA. After PCR with the TDP3 and MRT protocols and gel electrophoresis, products were electroblotted onto nylon membrane and hybridized with biotin-labeled HPV-16 oligonucleotide probe detected with streptavidin-alkaline phosphatase and NBT/BCIP. The data confirm the ~150 bp amplicons represent HPV sequences.
Evans et al. BMC Clinical Pathology 2005 5:10 doi:10.1186/1472-6890-5-10