Immunostaining of ICAM-1 expression in WT and P/I null mice. Tissues ICAM-1 expression was determined by staining the liver sections with an anti-ICAM-1 antibody specific to mouse by applying the immunoperoxidase technique, and examined under a light NIKON microscope. The top row represents wild-type (WT) control (CT), CLP 6 h and CLP 24 h, respectively. Note the increased intensity of ICAM-1 staining of central veins (arrow heads) and sinusoids (arrows) with progression of sepsis. In contrast, ICAM-1 expression in P/I null mice liver sections was completely absent in controls, 6 h after CLP and 24 h after CLP (lower row). Control group represents mice that were not subjected to sham or CLP experimental treatment.
Crockett et al. BMC Clinical Pathology 2004 4:2 doi:10.1186/1472-6890-4-2