Representative sections of p85 and PI3K-C2β (E, F and Q) expression in adult human tissues. In the GI tract, expression was observed in the lower third of gastric glands (A), in villous enterocytes (B), in surface colonocytes (C) and in macrophages infiltrating the lamina propria, especially in the appendix (D). Note the lack of expression of p85 in reactive germinal centres (GC) or in mature B lymphocytes. Myenteric plexuses and unmyelinated nerves (arrow heads) penetrating smooth muscles expressed PI3K-C2β as shown (E). PI3K-C2β was strongly expressed in alveolar macrophages in the lung (marked by arrows) (F). In the skin, p85 reactivity was observed in sebaceous glands (G), in hair follicles (H) and in Langerhans cells (I). Luminal p85 immunoreactivity, which was observed in endometrial glands in the proliferative phase (J) gradually disappeared when the secretory phase developed to remain only on the surface (K). In the cervix (L), and breast (O) only infiltrating macrophages expressed p85, serving as an internal positive control. In the ovarian follicle, p85 expression was seen in granulosa and theca cells (M). Columnar ciliated fallopian tube epithelium was strongly reactive for p85 (N). In the testicular seminiferous tubules, p85 was expressed in the cytoplasm (P) while PI3K-C2β was expressed in the nuclei (Q) of primary spermatocyes (PS), spermatids (Sp) but not in sertoli cells (S). Transitional epithelium p85 expression was intensified in the uppermost (umbrella) layer (R). Original magnification (A, B, G, J, N × 100), (C, D, F, H, L, M × 200), (E, F, I, K, O-R × 400).
El Sheikh et al. BMC Clinical Pathology 2003 3:4 doi:10.1186/1472-6890-3-4