Email updates

Keep up to date with the latest news and content from BMC Clinical Pathology and BioMed Central.

Open Access Research article

Evaluation in an emergency department of rapid separator tubes containing thrombin for serum preparation prior to hs-cTnT and CK-MB analyses

Yasemin U Budak1*, Kagan Huysal2, Mehtap Bulut3 and Murat Polat4

Author Affiliations

1 Department of Clinical Laboratory, Sevket Yilmaz Education and Research Hospital, Sevket Yilmaz Devlet Hastanesi. Biyokimya Laboratuari. Yildirim, Bursa, Turkey

2 Department of Clinical Laboratory, Yüksek İhtisas Education and Research Hospital, Bursa, Turkey

3 Department of Emergency Medicine, Sevket Yilmaz Education and Research Hospital, Bursa, Turkey

4 Department of General Surgery, Sevket Yilmaz Education and Training Hospital, Bursa, Turkey

For all author emails, please log on.

BMC Clinical Pathology 2013, 13:20  doi:10.1186/1472-6890-13-20

Published: 13 June 2013

Abstract

Background

In our emergency department, we collect blood in Rapid Serum Tubes (RSTs; Becton Dickinson, Franklin Lakes, NJ), in which clotting times are reduced. We investigated the influence of RST use on cardiac troponin T (hs-cTnT) and creatine kinase-MB (CK-MB) test results, in comparison with the use of tubes featuring a separator gel containing a clotting activator (SSTs; Green-vac, Yongin, Korea).

Methods

Samples from 60 patients were divided into equal aliquots and placed into RSTs and SSTs; hs-cTnT and CK-MB concentrations were determined using an autoanalyzer (Elecsys 2010) running commercial assays (Roche Diagnostics, Penzberg, Germany). Between-tube differences in CK-MB and hs-cTnT values were compared using the paired t-test, and correlations among variables were evaluated by calculation of Spearman correlation coefficients (r values). Deming regression analysis was performed and Bland-Altman plots were constructed.

Results

The hs-cTnT and CK-MB test results obtained from samples placed into RSTs and SSTs did not differ (p > 0.1). The correlations between the concentrations of hs-cTnT and CK-MB in samples placed into RSTs and SSTs were good; both r values were unity (p < 0.001). Deming regression analysis yielded the equation: RST [hs-cTnT] = 0.98 SST [hs-cTnT] + 0.69 pg/ml; and RST [CK-MB] = 0.95 SST [CK-MB]–0.09 ng/ml. The biases of 1.4 pg/ml (95% CI: minus 8.1–10.7 pg/ml) for hs-cTnT levels and 0.249 ng/ml (95% CI: minus 0.682–1.681 ng/ml) for CK-MB levels assayed using either tube was acceptable.

Conclusion

The hs-cTnT and CK-MB test results did not significantly differ when either tube was used. RST tube use was associated with a short clotting time; this was an advantage in an emergency laboratory setting.

Keywords:
Rapid serum tubes; Cardiac troponin T; Creatine kinase-MB