Table 3

Overall concordance analysis between manual and automated HER2 for three categorical HER2/CEP17 FISH parameters
Manual HER2 FISH (%) Automated HER2 FISH (%) Pearson chi 2 coefficient P value Concordance rate (%) Cohen’s kappa
HER2 genetic heterogeneity
Present (5-50% carcinoma cells with HER2/CEP17 ratio >2.2) 17 14 18.59 < 0.001 85 0.428
 Absent 83 86
HER2 cluster
 Present (HER2 cluster in >1% cells) 41 39 76.71 < 0.001 94 0.875
 Absent 59 61
CEP17 polyploidy
 Present (Chr. 17 copy number >3.00) 4 12 15.66 < 0.001 90 0.335
 Absent 96 88

Öhlschlegel et al.

Öhlschlegel et al. BMC Clinical Pathology 2013 13:13   doi:10.1186/1472-6890-13-13

Open Data