LINE-1 and Alu methylation patterns. The dark circles represent methylated cytosine, while the hollow circles represent unmethylated cytosine. There are four possible methylation patterns for the LINE-1 and Alu amplicons, including hypermethylated loci (mCmC), hypomethylated loci (uCuC), and 2 partially methylated loci (mCuC and uCmC). In each model, TaqI specifically identified methylated cytosine, while TasI specifically identified unmethylated cytosine. (A) The different methylation patterns of LINE-1 resulted in four differently sized digested products of 160 bp, 98 bp, 80 bp and 62 bp. (B) The different methylation patterns of the Alu element resulted in four differently sized digested products of 117 bp, 74/75 bp, 42/43 bp and 32 bp.
Sirivanichsuntorn et al. BMC Clinical Pathology 2013 13:10 doi:10.1186/1472-6890-13-10