Figure 1.

LAT1 and 4F2hc expression in human brain gliomas and normal brain tissues. Upper two panels (A-F) for LAT1 expression and lower two panels (G-L) for 4F2hc expression. (A) Pilocytic astrocytoma (WHO grade I) with few LAT1 staining microvessels (graded +). (B) Fibrillary astrocytoma (WHO grade II) with more LAT1 staining microvessels (graded ++). (C) Anaplastic astrocytoma (WHO grade III) with strong over 50% LAT1 staining in the cytoplasm and plasma membrane of tumor cells (graded +++). (D) Glioblastoma (WHO grade IV) with strong over 50% LAT1 staining in cytoplasm and plasma membrane of tumor cells and more LAT1 staining microvessels (graded +++). (E) Glioblastoma (WHO grade IV) with strong over 50% LAT1 staining in the cytoplasm and plasma membrane of tumor cells and abundant LAT1 staining microvessels (graded +++). (F) Normal brain tissue adjacent to the glioblastoma (D) with only negligible LAT1 staining. (G) Pilocytic astrocytoma (WHO grade I, the same specimen as shown in A) with weak over 50% F2hc staining in the cytoplasm and plasma membrane of tumor cells (graded +). (H) Gemistocytic astrocytoma (WHO grade II) with strong less/equal 50% 4F2hc staining in the cytoplasm and plasma membrane of tumor cells (graded ++). (I) Anaplastic astrocytoma (WHO grade III) with strong over 50% 4F2hc staining in the plasma membrane and cytoplasm of tumor cells (graded +++). (J) Glioblastoma (WHO grade IV) with strong over 50% 4F2hc staining in the cytoplasm and plasma membrane of tumor cells and more 4F2hc staining microvessels (arrow heads, graded +++). (K) Glioblastoma (WHO grade IV, the same specimen as shown in E) with strong over 50% 4F2hc staining in the cytoplasm and plasma membrane of tumor cells (graded +++). (L) Normal brain tissue adjacent to the glioblastoma (E and K) without obvious 4F2hc staining. Immunohistochemical polymer-peroxidase method was used, immunoreactions were visualized with 3,3'-diaminobenzidine tetrahydrochloride (DAB) and nuclear counterstaining was performed with Mayer's hematoxylin. Original magnification × 400, and all bars = 20 μm.