Table 2

Effect of frozen storage on the detection of bacterial OTUs by 16S rRNA-based T-RFLP

OTU (bp)

Log odds of detection

p value

95% CI

Closest Bacterial Match (>97% Sequence Similarity)


87

1.81

0.25

-1.25, 4.86

91

-3.04

0.01

-5.43, -0.65

Firmicutes

114

-2.00

0.04

-3.95, -0.05

Moraxella sp.

116

2.63*

0.36

-2.96, 8.22

Marinomonas sp.

133, 215

2.63*

0.36

-2.96, 8.22

155

1.34

0.20

-0.69, 3.38

216

-1.18

0.15

-2.80, 0.43

Corynebacterium propinquum, Rothia mucilaginosa

273

-2.44

0.26

-6.66, 1.79

Flavobacteriaceae

294

0.94*

0.52

-1.89, 3.76

309

-2.77

0.03

-5.34, -0.21

Pseudomonas sp.

392

-2.21

0.06

-4.52, 0.11

492

-0.67

0.57

-2.99, 1.66

Clostridiales Incertae Sedis XI

502

2.63*

0.36

-2.96, 8.22

504

-2.39

0.25

-6.49, 1.70

Haemophilus influenzae

518

-2.59

0.01

-4.58, -0.61

Haemophilus influenzae, Burkholderia fungorum, Comamonadaceae

520

-0.36

0.68

-2.02, 1.31

Staphylococcus sp.

521

-1.70

0.11

-3.80, 0.40


A random effects logistic regression model adjusting for sex was used. * Indicates OTUs for which sex adjustment was not done. Bold indicates significant results at the 5% level after correcting for multiple testing using Holm's method none of them appeared significant

Kwambana et al. BMC Clinical Pathology 2011 11:2   doi:10.1186/1472-6890-11-2

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