Table 1

Overview of sample processing

Parameter

source 1

source 2

source 3


Origin

AIT

Austrian Red Cross

AKH

Sample type

serum/plasma

serum

serum

Test persons

control individuals

age and sex matched

control individuals

age and sex matched

control/breast cancer

Quantity

serum, n = 12

plasma, n = 8

serum, n = 48

non-canc., n = 30

cancer met., n = 12

cancer mal., n = 30

cancer ben., n = 12

Isolation

MBD/silica*

MBD/silica*

MBD/silica**

Analyses

DNA quantification

multiplexed PCR test

qPCR measurments

array based autoanitbody

tests

DNA quantification

multiplexed PCR test

DNA quantification


AIT, Austrian Institute of Technology; AKH, Vienna General Hospital; cancer met., serum samples from cancer patients with a metastasizing tumor; cancer mal., patients with an malign tumor; cancer ben., derived from patient with a benign tumor; *DNA of all samples isolated by both isolation methods; ** DNA from 72 samples of this group was isolated using "silica", 12 using "MBD" MBD, methyl-binding domain protein; silica, silica membrane-based isolation

Wielscher et al. BMC Clinical Pathology 2011 11:11   doi:10.1186/1472-6890-11-11

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