Increased level of cell free DNA in serum of Breast cancer patients with metastasizing disease. Amount of cell free DNA isolated from 1 ml serum of breast cancer patients with metastasizing tumors and control individuals obtained from AKH (source 3). An increased serum DNA amount was detected in sera from metastasizing tumors with both isolation strategies. (A), silica based isolation protocol (P = 0.0043, Wilcoxon test); (B) MBD loaded bead based purification (P = 0.0021, Wilcoxon test).
Wielscher et al. BMC Clinical Pathology 2011 11:11 doi:10.1186/1472-6890-11-11