KRAS Mutation Detection Without Dissection. DNA was prepared from a G12 D KRAS-mutant colorectal cancer resection specimen with ~3% tumor cells by scraping the tissue from the entire slide without tumor dissection. The KRAS result was equivocal using regular-PCR (upper left panel), but a mutation was clearly detected using COLD-PCR (lower left panel). The corresponding HE-stained slide and region of tumor are depicted in the right panel.
Pritchard et al. BMC Clinical Pathology 2010 10:6 doi:10.1186/1472-6890-10-6