Figure 14.

Effect of short-chain polysaturated fatty acids (PFAs) homologous series (C3 – C9) on androgen-dependent AR transactivational capacity. The effect of short chain (C3 – C8) polysaturated fatty acids (PFAs) on androgen dependent AR transactivational capacity. Hela cells tranfected with AR expression plasmid were exposed to 10 μM of different PFAs as indicated, together with 1 nM DHT. C3 = propanoic acid (3:0). C4 = butanoic acid (4:0). C5 = pentanoic acid (5:0). C6 = n-caproic acid (6:0). C7 = heptanoic acid (7:0). C8 = caprylic acid (8:0). C9 = pelargonic acid (9:0). AR activity was measured with the multimeric AR reporter plasmid (ARE₂-TATA-Luc) and luciferase activity in relative light units (RLU) was mean ± SE of 3 replicates.