Table 3

Antimicrobial activity of 21 essential oils against P. vulgaris, P. aeruginosa and E. coli using disc diffusion method

Oil Name
P. vulgaris
P. aeruginosa
E. coli

Concentration
1:1
1:5
1:10
1:20
1:1
1:5
1:10
1:20
1:1
1:5
1:10
1:20

Aniseed oil
-
-
-
-
-
-
-
-
-
-
-
-
Calamus oil
11.1 ± 0.7gh
9.2 ± 0.5h
-
-
13.8 ± 0.5ef
12.8 ± 0.4f
9.2 ± 0.7 d
8.3 ± 0.5 d
-
-
-
-
Camphor oil
8.8 ± 0.7 hi
-
-
-
10 ± 0.5gh
-
-
-
8.8 ± 1d
-
-
-
Cedar wood
14.1 ± 1.1f
12.9 ± 0.5g
8.2 ± 0.5f
-
15.6 ± 0.5de
14.1 ± 0.9ef
8.23 ± 0.5d
-
-
-
-
-
Cinnamon oil
29.4 ± 0.5a
27 ± 0a
18.6 ± 1.52a
14.1 ± 0.4b
33.3 ± 1.6a
32.2 ± 0.5a
24.4 ± 0.5a
21 ± 0a
29.8 ± 0.7a
26 ± 1a
23.8 ± 1a
21 ± 0.2a
Citronella oil
19.5 ± 0.5cd
11.3 ± 0.7g
-
-
12.1 ± 0.5fg
10.4 ± 0.5g
-
-
-
-
-
-
Clove oil
20.1 ± 1c
18.2 ± 0.5d
11.8 ± 0.5d
8 ± 0e
17.4 ± 0.7d
15.5 ± 0.2e
8.5 ± 0.5d
-
17.4 ± 2c
15.1 ± 0.5c
10.8 ± 0.5e
8 ± 0c
Eucalyptus oil
-
-
-
-
-
-
-
-
8.5 ± 1d
-
-
-
Geranium oil
19 ± 0.2de
14.6 ± 0.2e
11 ± 1.5ef
8.3 ± 0e
21.5 ± 0.2c
19.2 ± 1.2d
9.4 ± 0.5d
-
14.1 ± 1c
10.4 ± 1d
-
-
Lavender
10.8 ± 0.5ghi
9.3 ± 0.5h
-
-
12.1 ± 0.9fg
9.9 ± 0.7g
-
-
-
-
-
-
Lemon oil
22.5 ± 0.5b
18.9 ± 0.5d
15.5 ± 0.5c
10.7 ± 0.2d
21.4 ± 1.1c
19.9 ± 0.7cd
9.1 ± 0.2d
-
21.6 ± 0.5b
18.9 ± 0.5b
10.6 ± 0.2e
-
Lemongrass oil
14.6 ± 0.7f
12.1 ± 0.2g
9.5 ± 0.5ef
-
23.4 ± 1c
19.6 ± 0.5d
9.1 ± 0.5d
-
-
-
-
-
Lime oil
27.8 ± 0.5a
24 ± 0b
17.9 ± 0.1ab
13 ± 1c
25.5 ± 1b
23.5 ± 0.5b
13.6 ± 0.7c
9.3 ± 0.8c
22.7 ± 0.5b
18.9 ± 1b
15.6 ± 0.5c
17.6 ± 1.5b
Nutmeg
14.5 ± 0.7f
11.9 ± 0.2g
8 ± 0 f
-
15.5 ± 0.5de
12.9 ± 1f
-
-
-
-
-
-
Orange oil
23.7 ± 0.7b
22.2 ± 0.7 c
16.5 ± 0.2bc
10.7 ± 0.5d
21.6 ± 0.2c
18.8 ± 0.2d
9.4 ± 0.5d
-
22.9 ± 0.5b
19.4 ± 0.7b
12.4 ± 0.2d
-
Palmarosa oil
19 ± 0cd
15.8 ± 0.2 e
9.9 ± 0.2 def
-
14.4 ± 0.5ef
13.2 ± 0.5ef
9.2 ± 0.2d
-
8.2 ± 0.5d
-
-
-
Peppermint oil
8.5 ± 0.2i
-
-
-
13.4 ± .52ef
11.2 ± 0.8g
-
-
-
-
-
-
Rosemary oil
17.4 ± 1cd
15.9 ± 0.5ef
9.7 ± 0de
-
23.4 ± 0.2c
21.9 ± 0.5c
14.5 ± 0.5b
8.3 ± 0.5d
17.5 ± 1c
15.1 ± 0.7c
9.1 ± 0.2f
-
Basil oil
8.9 ± 0.7ghi
-
-
-
8.23 ± 0.5h
-
-
-
10.5 ± 0.5d
8.2 ± 0.5e
-
-
Vetiver oil
11.5 ± 0.5g
10.3 ± 0.2
11.4 ± 0.5de
-
13.5 ± 0.5ef
11.7 ± 0.5g
8.2 ± 0.2d
-
-
-
-
-
Wintergreen
15.8 ± 0.7ef
14.8 ± 0.5 f
9.5 ± 1 def
-
10.2
-
-
-
8.9 ± 1d
-
-
-
Streptomycin**
18.4 ± 0.7



16.4 ± 0.2



21.2 ± 0.1



Vehicle control
-



-



-




** Streptomycin disc (25 μg) as a positive reference standard; Values are mean inhibition zone(mm) ± S.D of three replicates

Means within a column followed by different letters are statistically significant by tukey's test at p = 0.05; - no activity.

Prabuseenivasan et al. BMC Complementary and Alternative Medicine 2006 6:39   doi:10.1186/1472-6882-6-39