IGF-1 production from human cartilage explants in response to IL-1β, vincaria and RNI 249 and their combination. Control, untreated cartilage explants (n = 6) release a defined amount of IGF-1 into the bathing media. However, in IL-1β (5 ng/ml) treated explants IGF-1 levels were immeasurable (n = 6). Co-treatment with either vincaria 10 μg/ml (V10, n=3), or RNI 249 50 μg/ml (R50, n = 6), partially restored IGF-1 production. The combination of vincaria and RNI 249 (IL-1 + V + R, n = 3) produced additive effects that resulted in the restoration of IGF-1 levels to control values despite the presence of IL-1β. The * denotes a significant difference from all other groups (P < 0.01).
Miller et al. BMC Complementary and Alternative Medicine 2006 6:13 doi:10.1186/1472-6882-6-13