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Open Access Research article

Dai Huang Fu Zi Tang could ameliorate intestinal injury in a rat model of hemorrhagic shock by regulating intestinal blood flow and intestinal expression of p-VASP and ZO-1

Xiaoguang Lu1*, Xin Kang2, Libin Zhan3*, Chunyu Lv4, Zhiwei Fan1, Yingli Wang2, Robbie Ali5, Chang Lv6, Siyao Li4 and Jinhai Mu6

Author Affiliations

1 Department of Emergency Medicine, Zhongshan Hospital, Dalian University, Dalian 116001, China

2 College (Institute) of Integrative Medicine, Dalian Medical University, Dalian 116044, China

3 Department of Traditional Chinese Medicine, the Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China

4 Graduate School of Zunyi Medical University, Zunyi 563003, China

5 Windber Hospital Inc, Windber Medical Center, Windber, PA 15963, USA

6 Graduate School of Dalian University, Dalian 116044, China

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BMC Complementary and Alternative Medicine 2014, 14:80  doi:10.1186/1472-6882-14-80

Published: 1 March 2014

Abstract

Background

Dai Huang Fu Zi Tang (DHFZT), an oriental herbal formula, has long been used clinically in treatment of intestinal obstruction, acute pancreatitis, cholecystalgia and chronic diarrhea for thousands of years. Recent studies have demonstrated that DHFZT can reduce intestinal pathological injury and the concentration of enterogenous endotoxin in an animal model. But the underlying mechanism has not been fully elucidated.

Methods

SD male rats in adult were used to model HS and treated with DHFZT. The serum concentration of endotoxin were analyzed by dynamic turbidimetric method. In addition, the blood flow of small intestine were measured using laser speckle technique. Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP) and zonula occludens (ZO)-1 protein, intestinal fatty acid binding protein (IFABP) were measured by Western Blotting, RT-PCR, ELISA respectively.

Results

Present study showed that DHFZT markedly elevated the blood flow of small intestine, protected the intestinal barrier function by up-regulating the expression of ZO-1 protein and down-regulating expression of p-VASP, and notely decreased serum concentration of IFABP and endotoxin in HS.

Conclusions

These results reveal that DHFZT improves intestinal blood flow, protects the intestinal barrier function, and ameliorates intestinal endotoxaemia in rats with HS.

Keywords:
Hemorrhagic shock; Dai Huang Fu Zi Tang; Endotoxin; Small intestine; Phosphorylated vasodilator-stimulated phosphoprotein (p-VASP); Zonula occludens (ZO)-1 protein; Intestinal fatty acid binding protein (IFABP)