Anti-allergic effect of a Korean traditional medicine, Biyeom-Tang on mast cells and allergic rhinitis
- Equal contributors
1 Research and Development Division, Korea Promotion Institute for Traditional Medicine Industry, Gyeongsan 712-260, Republic of Korea
2 Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Yongin 449-728, Republic of Korea
3 Department of Clinical Pathology, Tae Kyeung College, Gyongsan 712-719, Republic of Korea
4 School of Medicine, Catholic University of Daegu, Daegu 705-718, Republic of Korea
BMC Complementary and Alternative Medicine 2014, 14:54 doi:10.1186/1472-6882-14-54Published: 12 February 2014
Biyeom-Tang, a medicine prescribed by oriental clinics, has been used for the treatment of the allergic rhinitis (AR). In the present study, an ethanol extract of Biyeom-Tang (EBT) was investigated for anti-allergic properties on bone-marrow derived mast cells (BMMC) and in vivo models.
The anti-allergic properties of EBT were evaluated by measuring β-Hex release and the production of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) on BMMC in vitro and PCA and OVA-induced AR models in vivo.
EBT strongly inhibited a degranulation reaction in a dose dependent manner with an IC50 value of 35.6 μg/ml. In addition, the generation of PGD2 and LTC4 was inhibited in BMMC in a concentration-dependent manner with IC50 values of 7.0 μg/ml and 10.9 μg/ml, respectively. When administrated orally, EBT ameliorated the mast cell-mediated PCA reaction. In the OVA-induced AR model, the increased levels of IgE were reduced by EBT. The levels of cytokines, such as IL-4, IL-5, IL-10, and IL-13 decreased in the splenocytes of EBT-treated mice. The histological analysis shows that the infiltration of inflammatory cells increased by OVA-sensitization was also reduced.
Taken together, these results suggested that EBT has anti-allergic and anti-inflammatory effects in vitro and in vivo models.