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Open Access Research article

Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae)

Meenu Katoch1*, Gurpreet Singh1, Sadhna Sharma2, Nidhi Gupta3, Payare Lal Sangwan3 and Ajit Kumar Saxena2

Author Affiliations

1 Microbial Biotechnology Department, Indian Institute of Integrative Medicine, Jammu, India

2 Cancer Pharmacology Department, Indian Institute of Integrative Medicine, Jammu, India

3 Natural Product Chemistry, Indian Institute of Integrative Medicine, Jammu 180001, India

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BMC Complementary and Alternative Medicine 2014, 14:52  doi:10.1186/1472-6882-14-52

Published: 11 February 2014

Abstract

Background

Endophytes, which reside in plant tissues, have the potential to produce novel metabolites with immense benefits for health industry. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell were investigated.

Methods

Endophytic fungi were isolated from the Bacopa monnieri. Extracts from liquid cultures were tested for cytotoxicity against a number of cancer cell lines using the MTT assay. Antimicrobial activity was determined using the micro dilution method.

Results

22% of the examined extracts showed potent (IC50 of <20 μg/ml) cytotoxic activity against HCT-116 cell line. 5.5%, 11%, 11% of the extracts were found to be cytotoxic for MCF-7, PC-3, and A-549 cell lines respectively. 33% extracts displayed antimicrobial activity against at least one test organism with MIC value 10–100 μg/ml. The isolate B9_Pink showed the most potent cytotoxic activity for all the cell lines examined and maximum antimicrobial activity against the four pathogens examined which was followed by B19.

Conclusions

Results indicated the potential for production of bioactive agents from endophytes of Bacopa monnieri.

Keywords:
Bacopa monnieri; Endophytic fungi; MTT assay; Antimicrobial activity; Cytotoxicity; HCT-116