Open Access Research article

Photoprotection of Buddleja cordata extract against UVB-induced skin damage in SKH-1 hairless mice

José Guillermo Avila Acevedo1*, Adriana Montserrat Espinosa González1, Diana Matamoros De Maria y Campos1, José del Carmen Benitez Flores2, Tzasna Hernández Delgado3, Saul Flores Maya4, Jorge Campos Contreras5, José Luis Muñoz López1 and Ana María García Bores1

Author Affiliations

1 Laboratorio de Fitoquímica, Unidad de Biología, Tecnología y Prototipos (UBIPRO), Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No. 1, Los Reyes Iztacala, Tlalnepantla, Edo. de México C.P. 54090, México

2 Laboratorio 1, UMF, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, Edo. de México, México

3 Laboratorio de Farmacognosia, UBIPRO, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, Edo. de México, México

4 Laboratorio de Recursos Naturales, UBIPRO, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, Edo. de México, México

5 Laboratorio de Bioquímica Molecular, UBIPRO, Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, Edo. de México, México

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BMC Complementary and Alternative Medicine 2014, 14:281  doi:10.1186/1472-6882-14-281

Published: 3 August 2014

Abstract

Background

In recent years, there has been considerable interest in using botanical agents to prevent skin damage resulting from solar UV-irradiation. Buddleja cordata is a plant that is known as “tepozan”. Some people in Mexico use the leaves of this plant to treat tumours, abscesses, sores and burns. The purpose of this study is to investigate the photoprotective properties of Buddleja cordata methanolic extract (BCME) against UVB-induced skin damage in SKH-1 hairless mice at the macroscopic and histological levels.

Methods

BCME was characterised to determine its spectroscopic, chromatographic and antioxidant (DPPH, superoxide and hydroxyl radicals) properties. To conduct the photoprotection studies, BCME was applied topically to the skin of SKH-1 mice before acute exposure to UVB for 10 minutes. The murine skin samples were used for macroscopic and histological studies to assess tissue damage. Penetration of active components of BCME into stratum corneum on the dorsal area of mice was investigated in vivo by the tape stripping method. Moreover, genotoxicity of BCME was evaluated in a Vicia faba cell root micronucleus model.

Results

BCME displayed absorbance over the entire UVB spectrum, and its principal components included verbascoside and linarin. BCME exhibited antioxidant activity and significantly scavenged hydroxyl radicals. BCME reduced erythema, sunburn cell production, vessel congestion and epidermal thickening of UVB irradiated mouse skin. BCME penetrate the skin of mice. BCME did not exhibit genotoxic activity in the micronucleus test.

Conclusion

The topical administration of BCME protected against acute UVB-induced damage in mouse SKH-1 skin, and our results suggest that BCME may potentially prevent photodamage.

Keywords:
Buddleja cordata; Phenolics; Antioxidant; UV radiation; Sunburn; Erythema; Photoprotection