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Open Access Research article

Anti-inflammatory effects of sargachromenol-rich ethanolic extract of Myagropsis myagroides on lipopolysaccharide-stimulated BV-2 cells

Sunghee Kim1, Min-Sup Lee14, Bonggi Lee2, Wi-Gyeong Gwon1, Eun-Ji Joung1, Na-Young Yoon3 and Hyeung-Rak Kim1*

Author Affiliations

1 Department of Food Science and Nutrition, Pukyong National University, Yongso-ro, Nam-gu, Busan 608-737, South Korea

2 National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA

3 Food and Safety Research Division, National Fisheries Research and Development Institute, 216, Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 619-705, South Korea

4 Institute of Fisheries Sciences, Pukyong National University, Ilgwang-ro, Ilgwang-myeon, Gijang-gun, Busan 619-911, South Korea

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BMC Complementary and Alternative Medicine 2014, 14:231  doi:10.1186/1472-6882-14-231

Published: 9 July 2014

Abstract

Background

Excessive pro-inflammatory cytokine production from activated microglia contributes to neurodegenerative diseases, thus, microglial inactivation may delay the progress of neurodegeneration by attenuating the neuroinflammation. Among 5 selected brown algae, we found the highest antioxidant and anti-neuroinflammatory activities from Myagropsis myagroides ethanolic extract (MME) in lipopolysaccharide (LPS)-stimulated BV-2 cells.

Methods

The levels of nitric oxide (NO), prostaglandin E2 (PGE2), and pro-inflammatory cytokines were measured by Griess assay and enzyme linked immunesorbent assay. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinases (MAPKs), and Akt were measured using Western blot. Nuclear translocation and transcriptional activation of nuclear factor-κB (NF-κB) were determined by immunefluorescence and reporter gene assay, respectively.

Results

MME inhibited the expression of iNOS and COX-2 at mRNA and protein levels, resulting in reduction of NO and PGE2 production. As a result, pro-inflammatory cytokines were reduced by MME. MME also inhibited the activation and translocation of NF-κB by preventing inhibitor κB-α (IκB-α) degradation. Moreover, MME inhibited the phosphorylation of extracellular signal regulated kinases (ERKs) and c-Jun N-terminal kinases (JNKs). Main anti-inflammatory compound in MME was identified as sargachromenol by NMR spectroscopy.

Conclusions

These results indicate that the anti-inflammatory effect of sargachromenol-rich MME on LPS-stimulated microglia is mainly regulated by the inhibition of IκB-α/NF-κB and ERK/JNK pathways.

Keywords:
Myagropsis myagroides; Sargachromenol; Pro-inflammatory cytokine; Microglia; Nuclear factor-κB; Neuroinflammation