Selective apoptotic cell death effects of oral cancer cells treated with destruxin B
- Equal contributors
1 School of Nutrition, Chung Shan Medical University, Taichung, Taiwan
2 Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan
3 School of Medicine, Chung Shan Medical University, Taichung, Taiwan
4 Division of Pulmonary Medicine, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan
5 Department of Neurology, Chung Shan Medical University Hospital, Taichung, Taiwan
6 School of Medical Laboratory and Biotechnology, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, Taichung 40201, Taiwan
7 Department of Pathology, Chung Shan Medical University Hospital, Chung Shan Medical University, Taichung, Taiwan
8 Department of Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan
9 Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, 168 Jifong East Road, Wufong District, Taichung 41349, Taiwan
BMC Complementary and Alternative Medicine 2014, 14:207 doi:10.1186/1472-6882-14-207Published: 28 June 2014
Recent studies have revealed that destruxins (Dtx) have potent cytotoxic activities on individual cancer cells, however, data on oral cancer cells especial human are absent.
Destruxin B (DB) was isolated and used to evaluate the selective cytotoxicity with human oral cancer cell lines, GNM (Neck metastasis of gingival carcinoma) and TSCCa (Tongue squamous cell carcinoma) cells, and normal gingival fibroblasts (GF) were also included as controls. Cells were tested with different concentrations of DB for 24, 48, and 72 h by MTT assay. Moreover, the mechanism of cytotoxicity was investigated using caspase-3 Immunofluorescence, annexin V/PI staining, and the expression of caspase-3, Bax, and Bcl-2 by western blotting after treated with different concentrations of DB for 72 h as parameters for apoptosis analyses.
The results show that DB exhibited significant (p < 0.01) and selective time- and dose-dependent inhibitory effects on GNM and TSCCa cells viability but not on GF cells. The data suggested that DB is capable to induce tumor specific growth inhibition in oral GNM and TSCCa cancer cells via Bax/Bcl-2-mediated intrinsic mitochondrial apoptotic pathway in time- and dose-dependent manners.
This is the first report on the anti-proliferation effect of DB in oral cancer cells. The results reported here may offer further evidences to the development of DB as a potential complementary chemotherapeutic target for oral cancer complications.