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Open Access Research article

Increasing effect of Tangzhiqing formula on IRS-1-dependent PI3K/AKT signaling in muscle

Jing Gao1, Jian Li2, Yating An1, Xuefeng Liu3, Qian Qian3, Yanlin Wu2, Yi Zhang1* and Tao Wang2*

Author Affiliations

1 Tianjin State Key Laboratory of Modern Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China

2 Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 312 Anshanxi Road, Nankai District, Tianjin 300193, China

3 Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Ministry of Education, 312 Anshanxi Road, Nankai District, Tianjin 300193, China

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BMC Complementary and Alternative Medicine 2014, 14:198  doi:10.1186/1472-6882-14-198

Published: 21 June 2014

Abstract

Background

Tangzhiqing fomula (TZQ-F), the mixture of Red Paeony root, Mulberry leaf, Lotus leaf, Danshen root and Hawthorn leaf, regulates the abnormal glucose and lipids in prediabetic patients. In this study, we focus on the mechanism of TZQ-F and its fractions on glucose metabolism.

Methods

After orally administration of TZQ-F for 4 weeks in KK-Ay mice, we dissected out the liver and muscle, and employed PCR and western blotting to screening the PI3K/AKT pathway. The following PI3K/AKT signaling pathway were performed in L-6 myotube and HepG2 cells.

Results

In the liver of KK-Ay mice, no significance was observed on PI3K, AKT and their phosphorylation between TZQ-F and controls , while, in the muscle, up-regulation of PI3K, AKT, Glycogen synthase (GYS) and their phosphorylation type, as well as GluT4, was deteced in TZQ-F. In HepG2 cells, TZQ-F increased IRS-2 by 10 folds, without interrupting AKT, IRS-1 and GluT4. In L-6 myotube cells, TZQ-F and its fractions treatment significantly increased IRS-1 and AKT at mRNA level.

Conclusion

TZQ-F prevents pre-diabetes through increasing effect on IRS-1-dependent PI3K/AKT signaling pathway in muscle.

Keywords:
IRS-1-dependent PI3K signaling pathway; Insulin resistance; Paeonia lactiflora; Morus alba; Nelumbo nucifera; Salvia miltiorrhiza; Crataegus pinnatifida