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In vitro and ex-vivo cellular antioxidant protection and cognitive enhancing effects of an extract of Polygonum minus Huds (Lineminus) demonstrated in a Barnes Maze animal model for memory and learning

Annie George1*, Chee Perng Ng2, Matthew O’Callaghan2, Gitte S Jensen3 and Hoi Jin Wong1

Author Affiliations

1 Biotropics Malaysia Berhad, Lot 21, Jalan U1/19, Section U1, Hicom-Glenmarie Industrial Park, Shah Alam, Selangor 40150, Malaysia

2 Cerca Insights Sdn Bhd (NCIA Technology Development Center) Level 2, Plot No.36 Hilir Sungai Keluang, Bayan Lepas Industrial EstatePhase IV, Bayan Lepas, Penang 11900, Malaysia

3 NIS Labs, 1437 Esplanade, Klamath Falls, OR 97601, USA

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BMC Complementary and Alternative Medicine 2014, 14:161  doi:10.1186/1472-6882-14-161

Published: 19 May 2014



Polygonum minus a culinary flavouring that is common in South East Asian cuisine and as a remedy for diverse maladies ranging from indigestion to poor eyesight. The leaves of this herb have been reported to be high in antioxidants. Flavonoids which have been associated with memory, cognition and protection against neurodegeneration were found in P. minus.


This study examined a P. minus aqueous extract (Lineminus) for its antioxidant activity using the Oxygen Radical Absorbance Capacity (ORAC) assay, the ex vivo Cellular Antioxidant Protection of erythrocytes (CAP-e) assays and for potential anticholinesterase activity in vitro. Cognitive function and learning of Lineminus was evaluated using scopolamine induced cognition deficits in a Barnes maze, rodent model of cognition.


The extract displayed in vitro antioxidant activity with a total ORAC value of 16,964 μmole TE/gram. Cellular antioxidant protection from free radical damage using the CAP-e assay, with an IC50 of 0.58 g/L for inhibition of cellular oxidative damage, was observed. The extract inhibited cholinesterase activity with an IC50 of 0.04 mg/ml with a maximum inhibition of 68%. In a rodent model of cognition using scopolamine induced cognition deficits in the Barnes maze, the extract attenuated scopolamine induced disruptions in learning at the higher dose of 100 mg/kg.


These data shows that P. minus possesses antioxidant and anticholinesterase activity and demonstrated enhanced cognition in vivo. The data suggest neuroprotective properties of the extract.

Scopolamine; Antioxidant; Barnes maze; Polygonum minus; Cognition