Table 2

Effects of Polygonum viviparum (PV) on nitric oxide (NO) production by human umbilical vein endothelial cells (HUVECs)
Nitrite [μM]/5 × 105 cells
Blank PV (30 μg/ml) ACh (30 μM)
Blank 0.378 ± 0.559 8.445 ± 0.878 7.412 ± 0.661
L-NAME (50 μM) 0.631 ± 0.518 2.073 ± 0.244** 1.066 ± 0.165##
L-NMMA (50 μM) 0.476 ± 0.483 2.232 ± 0.383** 1.154 ± 0.397##
Ca2+-free 0.505 ± 0.442 0.668 ± 0.324** 0.964 ± 0.352##
BAPTA-AM (20 μM) 0.432 ± 0.378 0.584 ± 0.460** 0.511 ± 0.369##

HUVECs were incubated with PV and acetylcholine (ACh) for 1 h, and then the culture medium was collected for NO detection with the Griess reagent. Ca2+-free medium contained 2.5 mM EGTA. BAPTA-AM chelates intracellular Ca2+. Data are expressed as the mean ± standard error of the mean (S.E.M) for more than 5 individual experiments. ** p < 0.01 indicates a significant difference from PV alone. ##p < 0.01 indicates a significant difference from ACh alone.

Chang et al.

Chang et al. BMC Complementary and Alternative Medicine 2014 14:150   doi:10.1186/1472-6882-14-150

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