Table 1

Effects of Polygonum viviparum (PV) on cyclic guanosine 3′, 5′-monophosphate (cGMP) formation by the rat aorta
cGMP (pmol/mg protein)
Intact Denuded L-NAME 50 μM MB 10 μM Ca2+ free medium
Control 0.53 ± 0.08
SNP 10 μM 3.45 ± 0.56*
ACh 10 μM 3.21 ± 0.42* 0.60 ± 0.18 0.70 ± 0.09 0.52 ± 0.12 0.45 ± 0.11
PV 100 μg/ml 3.18 ± 0.74* 0.57 ± 0.13 0.54 ± 0.09 0.66 ± 0.07 0.64 ± 0.04

After pretreatment with 10 μM IBMX for 5 min, aortic segments were replaced in Ca2+-free Krebs’ (2.5 mM EGTA) buffer or treatment with inhibitors of L-NAME and methylene blue (MB) for 10 min and then sodium nitroprusside (SNP), acetylcholine (ACh) and PV were added for another 2 min. The aortic rings were immersed in liquid nitrogen to stop the reaction. cGMP formation were measured. Data are expressed as the mean ± standard error of the mean (S.E.M) of more than 5 individual experiments. –, not determined. * p < 0.05 indicates a significant difference from the control.

Chang et al.

Chang et al. BMC Complementary and Alternative Medicine 2014 14:150   doi:10.1186/1472-6882-14-150

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