Table 1

Genes up- and down-regulated for at least two of four S. aureus strains following a 30-min exposure to the cranberry product NC90 (2 mg/mL)
ORF SACOL Gene name Gene annotation Fold expressiona for each strain
ATCC 29213 MRSA N315 SHY97 4023 SHY97 3906 DAPb VANb OXAb OBCc Vrad
Up-regulated genes
0263 Peptidoglycan hydrolase 15.0 24.5 3.6 9.8 + = = =
1066 fmt Fmt protein 6.8 3.8 2.9 2.0 + + + + +
1459 Methionine sulfoxide reductase 2.3 6.0 −1.5 1.2 = + + +
1490 pbp2 Penicillin binding protein 2 2.5 2.8 1.0 1.9 + + + + +
1777 htrA Putative serine protease Htra 4.6 8.9 1.0 2.1 + + + =
1932 Transglycosylase domain protein 8.5 6.8 2.2 2.5 + = = + +
1942 vraR DNA-binding response regulator 8.8 10.9 nd 2.7 + + + = +
1943 vraS Sensor histidine kinase VraS 14.2 10.5 7.6 4.0 + + + + +
1944 Hypothetical protein 5.1 7.5 1.3 1.4 + + + + +
2017 groES Chaperonin nd 2.2 2.3 2.2 + + + =
2116 murAB UDP-N-AcGlc-1-carbotransferase 4.0 7.6 2.5 2.6 + + + + +
2291 Staphyloxanthin biosynthesis 6.0 2.2 4.5 3.9 =
2352 tcaA TcaA protein 5.1 6.3 1.1 1.4 + + + + +
2584 isaA Immunodominant antigen A 2.1 1.8 2.3 2.1 = = = =
Down-regulated genes
0095 spa IgG binding protein −33.3 −2.0 −37.5 nd = =
0096 sarS Accessory regulator −2.0 −2.9 −2.7 −2.2
0136-0150e capA-O Capsular polysaccharide −4.9 nd −3.1 −1.9 =
0317 Lipase precursor −8.6 −6.8 −4.1 −1.7 =
451-452e ahpFC Alkyl hydroperoxide reductase −3.4 −3.6 −8.2 −1.2 = =
0679 Na+/H+ antiporter, MnhA −4.5 −10.0 −13.0 −25.0 = = =
0688-0690e mntABC ABC transporter −43.5 −66.7 −86.7 −91.7 = =
0765-0766e saeSR Sensor/Histidine kinase −1.5 −3.7 −1.5 −3.6 = = =
1072 folD THF dehydrogenase −2.1 −2.6 −2.7 nd = = =
1079 purF amidophosphoribosyltransferase nd −10.0 −0.8 −2.6 =
1140 isdA Heme-iron transport −5.3 −2.1 −2.9 nd = =
1158-1160e sdhABC Succinate dehydrogenase −5.1 −7.9 −4.5 −3.8 =
1173 hla Alpha-hemolysin −3.6 −4.3 nd −2.6 = =
1262-1263e sucCD Succinyl-CoA synthetase −5.6 −3.1 −6.4 −2.3 = = =
1328 glnR Glutamine synthetase repressor −3.7 −6.3 −3.4 −5.3 =
1329 femC Glutamine synthetase FemC −2.7 −4.2 −3.2 −2.9 = =
1368 katA Catalase −3.2 −2.4 −5.2 −2.1 = + =
1385 acnA Aconitate hydratase −3.4 −3.0 −6.3 nd = = =
1396 fmtC FmtC protein −0.9 −2.4 −2.0 nd
1448-1449e sucAB 2-oxoglutarate dehydrogenase −8.1 −5.4 −4.8 −4.9 = = =
1741 icd Isocitrate dehydrogenase, NADP −5.6 −2.2 −11.5 −2.5 = = +
1742 gtlA Citrate synthase −6.7 −2.3 −14.3 −2.2 = = +
1743 Amino acid permease −3.1 −1.2 −4.8 −1.5 = = =
2055 rsbW Anti-sigma B factor −2.6 nd −1.6 −2.1 =
2057 rsbU Sigma factor B regulator protein −1.3 −3.1 −0.9 −2.1 = =
2066-2068e kdpABC Potassium-transporting ATPase −3.5 nd −2.3 −1.7 = = =
2293 NAD/NADP dehydrogenase −2.0 −2.4 −1.3 nd =
2694 geh lipase −27.3 −4.3 −20.0 nd = = =

a Data are expressed in log2 ratios of Cy5(cranberry-treated)/Cy3(untreated control); see material and methods.

b Up (+) or down (−) expression of the indicated gene by exposure to daptomycin (DAP), vancomycin (VAN) or oxacillin (OXA), as reported by Muthaiyan et al. [29]; the equal sign (=) indicates genes that were not affected.

c Up (+) or down (−) expression of the indicated gene by exposure to oxacillin (O), bacitracin (B) and D-cycloserine (C) as reported by Utaida et al. [30]; the equal sign (=) indicates genes that were not affected.

d The plus sign (+) indicates genes known to be regulated by the two-component response regulator VraSR following a cell wall stress [42].

e To avoid repetition, the fold modulation of genes arranged in operons is represented as an average for the indicated gene set.

Diarra et al.

Diarra et al. BMC Complementary and Alternative Medicine 2013 13:90   doi:10.1186/1472-6882-13-90

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