Effect of resveratrol on the growth of mammary fat pad tumors. 1 × 106 GFP-tagged MDA-MB-231 or MDA-MB-435 cells in Matrigel:DMEM (1:1) were inoculated at the mammary fat pad of female SCID (MDA-MB-231) or athymic nude mice (MDA-MB-435). One week following injection, mice were fed vehicle (Veh) or 0.5, 5, or 50 mg/kg BW resveratrol (Res) 5 times a week by oral gavage. Whole body fluorescence images were acquired once a week. Relative tumor growth was calculated as the integrated density of fluorescence on each day of imaging as a function of the integrated density of fluorescence of the same tumor on day 1 of treatment administration. (A), Average relative GFP-MDA-MB-231 tumor growth as a function of days. (B), Representative images of GFP-MDA-MB-231 tumors following vehicle or Res diets at day 108. (C), Relative GFP-MDA-MB-231 tumor growth for each treatment group at day 108 ±SEM. (D), Average relative GFP-MDA-MB-435 tumor growth as a function of days. (E), Representative images of GFP-MDA-MB-435 tumors following vehicle or Res diets at day 44. (F), Relative GFP-MDA-MB-435 tumor growth for each treatment group at day 44 ±SEM. Differences between means were determined using Student’s t-Test or one-way ANOVA with Dunnett's Multiple Comparison Test.
Castillo-Pichardo et al. BMC Complementary and Alternative Medicine 2013 13:6 doi:10.1186/1472-6882-13-6