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Open Access Research article

The inhibitory effect of tongxieyaofang on rats with post infectious irritable bowel syndrome through regulating colonic par-2 receptor

Xuguang Hu1*, Xiaojun Zhang2, Bin Han1 and Weijian Bei1

Author Affiliations

1 School of Traditional Chinese medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, P.R China

2 School of Pharmacy, Guangzhou University of Chinese Medicine, Guangzhou, P.R China

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BMC Complementary and Alternative Medicine 2013, 13:246  doi:10.1186/1472-6882-13-246

Published: 2 October 2013

Abstract

Background

The aims of this study were to evaluate the effect and mechanism of a traditional Chinese medicine formula: Tongxieyaofang (TXYF) on Rats with Post Infectious Irritable Bowel Syndrome (PI-IBS).

Methods

SD male rats in adult were used to model PI-IBS and treated with TXYF at three dosage for 14 consecutive days, and then visceral sensation and the frequency of stool in PI-IBS rats were investigated. In addition, the contents of SP, TNF- α and IL-6 in colonic mucosal were analyzed by ELISA. Moreover faecal serine protease activity and PAR-2 mRNA expression were measured by ultraviolet spectrophotometry and RT-PCR, respectively.

Results

Our study showed that TXYF attenuated visceral hyperalgesia and inhibited stool frequency in Campylobacter-stimulated Post Infectious Irritable Bowel Syndrome (PI-IBS) rats. Furthermore, TXYF decreased the colonic SP, TNF- α and IL-6 content in PI-IBS rats. In addition, the up-regulated colonic mucosa PAR-2 mRNA expression in PI-IBS rats was significantly suppressed by orally TXYF.

Conclusions

TXYF attenuated PI-IBS symptom by attenuating behavioral hyperalgesia and anti-diarrhea, the underlying mechanism was mediated by inhibiting PAR-2 receptor expression, reducing the levels of SP, TNF- α and IL-6 in colonic mucosa and decreasing faecal serine protease activity.

Keywords:
Irritable bowel syndrome; Tong-Xie-Yao-Fang; PAR-2 receptor