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Antioxidant, anti-adipocyte differentiation, antitumor activity and anthelmintic activities against Anisakis simplex and Hymenolepis nana of yakuchinone A from Alpinia oxyphylla

Rong-Jyh Lin1, Chuan-Min Yen1, Tzung-Han Chou2, Feng-Yu Chiang3, Guey-Horng Wang4, Ya-Ping Tseng5, Lin Wang6, Ting-Wei Huang7, Hui-Chuan Wang8, Leong-Perng Chan39*, Hsiou-Yu Ding8* and Chia-Hua Liang4*

Author Affiliations

1 Department of Parasitology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

2 Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan

3 Department of Otolaryngology-Head and Neck Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan

4 Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

5 Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan

6 Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan

7 Department of Public Health, Kaohsiung Medical University, Kaohsiung, Taiwan

8 Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

9 Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

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BMC Complementary and Alternative Medicine 2013, 13:237  doi:10.1186/1472-6882-13-237

Published: 26 September 2013



Alpinia oxyphylla is a common remedy in traditional Chinese medicine. Yakuchinone A is a major constituent of A. oxyphylla and exhibits anti-inflammatory, antitumor, antibacterial, and gastric protective activities.


Antioxidant and antitumor characteristics of yakuchinone A in skin cancer cells as well as novel mechanisms for the inhibition of adipocyte differentiation, cestocidal activities against Hymenolepis nana adults, and nematocidal activities against Anisakis simplex larvae are investigated.


Yakuchinone A presents the ability of the removal of DPPH·and ABTS+ free radicals and inhibition of lipid peroxidation. Yakuchinone A suppresses intracellular lipid accumulation during adipocyte differentiation in 3 T3-L1 cells and the expressions of leptin and peroxisome proliferator-activated receptor γ (PPARγ). Yakuchinone A induces apoptosis and inhibits cell proliferation in skin cancer cells. The inhibition of cell growth by yakuchinone A is more significant for non-melanoma skin cancer (NMSC) cells than for melanoma (A375 and B16) and noncancerous (HaCaT and BNLCL2) cells. Treatment BCC cells with yakuchinone A shows down-regulation of Bcl-2, up-regulation of Bax, and an increase in cleavage poly (ADP-ribose) polymerase (PARP). This suggests that yakuchinone A induces BCC cells apoptosis through the Bcl-2-mediated signaling pathway. The anthelmintic activities of yakuchinone A for A. simplex are better than for H. nana.


In this work, yakuchinone A exhibits antioxidative properties, anti-adipocyte differentiation, antitumor activity, and anthelmintic activities against A. simplex and H. nana.

Yakuchinone A; Antioxidant; Adipogenesis; Apoptosis; Hymenolepis nana; Anisakis simplex