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Open Access Research article

Antioxidant, anti-adipocyte differentiation, antitumor activity and anthelmintic activities against Anisakis simplex and Hymenolepis nana of yakuchinone A from Alpinia oxyphylla

Rong-Jyh Lin1, Chuan-Min Yen1, Tzung-Han Chou2, Feng-Yu Chiang3, Guey-Horng Wang4, Ya-Ping Tseng5, Lin Wang6, Ting-Wei Huang7, Hui-Chuan Wang8, Leong-Perng Chan39*, Hsiou-Yu Ding8* and Chia-Hua Liang4*

Author Affiliations

1 Department of Parasitology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

2 Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan

3 Department of Otolaryngology-Head and Neck Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan

4 Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

5 Institute of Basic Medical Sciences, National Cheng Kung University, Tainan, Taiwan

6 Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan

7 Department of Public Health, Kaohsiung Medical University, Kaohsiung, Taiwan

8 Institute of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan, Taiwan

9 Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

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BMC Complementary and Alternative Medicine 2013, 13:237  doi:10.1186/1472-6882-13-237

Published: 26 September 2013

Abstract

Background

Alpinia oxyphylla is a common remedy in traditional Chinese medicine. Yakuchinone A is a major constituent of A. oxyphylla and exhibits anti-inflammatory, antitumor, antibacterial, and gastric protective activities.

Methods

Antioxidant and antitumor characteristics of yakuchinone A in skin cancer cells as well as novel mechanisms for the inhibition of adipocyte differentiation, cestocidal activities against Hymenolepis nana adults, and nematocidal activities against Anisakis simplex larvae are investigated.

Results

Yakuchinone A presents the ability of the removal of DPPH·and ABTS+ free radicals and inhibition of lipid peroxidation. Yakuchinone A suppresses intracellular lipid accumulation during adipocyte differentiation in 3 T3-L1 cells and the expressions of leptin and peroxisome proliferator-activated receptor γ (PPARγ). Yakuchinone A induces apoptosis and inhibits cell proliferation in skin cancer cells. The inhibition of cell growth by yakuchinone A is more significant for non-melanoma skin cancer (NMSC) cells than for melanoma (A375 and B16) and noncancerous (HaCaT and BNLCL2) cells. Treatment BCC cells with yakuchinone A shows down-regulation of Bcl-2, up-regulation of Bax, and an increase in cleavage poly (ADP-ribose) polymerase (PARP). This suggests that yakuchinone A induces BCC cells apoptosis through the Bcl-2-mediated signaling pathway. The anthelmintic activities of yakuchinone A for A. simplex are better than for H. nana.

Conclusions

In this work, yakuchinone A exhibits antioxidative properties, anti-adipocyte differentiation, antitumor activity, and anthelmintic activities against A. simplex and H. nana.

Keywords:
Yakuchinone A; Antioxidant; Adipogenesis; Apoptosis; Hymenolepis nana; Anisakis simplex