Calcarea carbonica restores tumor-induced depletion of T cell populations. Lymphocytes from thymus, spleen, lymph-node and peripheral circulation of untreated or placebo-/calcarea carbonica-treated tumor-bearing mice were analyzed flow cytometrically to quantitate percentages of CD4+/CD8+ populations. (A) Flow cytometric dot plot shows CD4+ and CD8+ T cell population in the draining lymph node of untreated or placebo-/calcarea carbonica-treated tumor-bearing mice. (B) Graphical representation of flow cytometric data showing percentages of CD4+ and CD8+ T cells in thymus, spleen, lymph-node and peripheral circulation of untreated or placebo-/calcarea carbonica-/IL2-treated tumor-bearing mice. (C) Percent CD4+ and CD8+ T-cell apoptosis (Annexin-V-positivity) from the same experimental sets were determined flow cytometrically at 21 day of drug treatment. The values are mean ± SEM of five independent experiments. *p < 0.05 and **p < 0.001 when compared with respective non-tumor/tumor-bearing control sets and placebo/drug-treated sets.
Saha et al. BMC Complementary and Alternative Medicine 2013 13:230 doi:10.1186/1472-6882-13-230