Effect of Payena dasyphylla EA fraction on MMP-13 protein expression in IL-1β-stimulated NHAC-kn cells. The blot image (A) shown above represents results from three independent experiments while (B) shows the histogram of MMP-13 protein expression from the same experiment. C; Basal level of MMP-13 expression without IL-1β treatment and the rest are cells stimulated with 10 ng/mL human IL-1β and treated with increasing concentrations of sample treatment for 24 h. Conditioned-medium were assayed for pretein expression by using Western blotting. ***P < 0.001 and **P < 0.01 significantly different from IL-1β treated control group.
Citalingam et al. BMC Complementary and Alternative Medicine 2013 13:213 doi:10.1186/1472-6882-13-213