Effect of Payena dasyphylla EA bark fraction on the HYAL2 gene expression in IL-1β stimulated NHAC-kn cells. The gel image (A) shows the reverse transcriptase-PCR result represented from three independent experiments, while (B) shows the histogram of HYAL2 gene expression from the same experiment. C; Basal level of HYAL2 gene expression without IL-1β treatment and the rest are cells stimulated with 100 ng/ml human IL-1β with increasing concentrations of sample treatment for 6 hours. Apigenin (250 μM) was used as the positive control. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene expression was used as the loading control. ***P < 0.001, **P < 0.01 and *P < 0.05 significantly different from IL-1β treated control group.
Citalingam et al. BMC Complementary and Alternative Medicine 2013 13:213 doi:10.1186/1472-6882-13-213