Email updates

Keep up to date with the latest news and content from BMC Complementary and Alternative Medicine and BioMed Central.

Open Access Research article

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Carla de Campos Ciccone1, Denise Cristina Zuzzi1, Lia Mara Grosso Neves1, Josué Sampaio Mendonça1, Paulo Pinto Joazeiro2 and Marcelo Augusto Marretto Esquisatto1*

Author Affiliations

1 Programa de Pós-graduação em Ciências Biomédicas, Centro Universitário Hermínio Ometto, Av. Dr. Maximiliano Baruto, 500 Jd. Universitário, 13607-339, Araras, SP, Brazil

2 Departamento de Histologia e Embriologia, Instituto de Biologia, Universidade Estadual de Campinas, Rua Charles Darwin, s/n. CxP 6109, 13083-863, Campinas, SP, Brazil

For all author emails, please log on.

BMC Complementary and Alternative Medicine 2013, 13:17  doi:10.1186/1472-6882-13-17

Published: 19 January 2013



In this study, we investigate the effects of microcurrent stimulation on the repair process of xiphoid cartilage in 45-days-old rats.


Twenty male rats were divided into a control group and a treated group. A 3-mm defect was then created with a punch in anesthetized animals. In the treated group, animals were submitted to daily applications of a biphasic square pulse microgalvanic continuous electrical current during 5 min. In each application, it was used a frequency of 0.3 Hz and intensity of 20 μA. The animals were sacrificed at 7, 21 and 35 days after injury for structural analysis.


Basophilia increased gradually in control animals during the experimental period. In treated animals, newly formed cartilage was observed on days 21 and 35. No statistically significant differences in birefringent collagen fibers were seen between groups at any of the time points. Treated animals presented a statistically larger number of chondroblasts. Calcification points were observed in treated animals on day 35. Ultrastructural analysis revealed differences in cell and matrix characteristics between the two groups. Chondrocyte-like cells were seen in control animals only after 35 days, whereas they were present in treated animals as early as by day 21. The number of cuprolinic blue-stained proteoglycans was statistically higher in treated animals on days 21 and 35.


We conclude that microcurrent stimulation accelerates the cartilage repair in non-articular site from prepuberal animals.

Hyaline cartilage; Tissue repair; Extracellular matrix; Electrotherapy; Immature rats