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Open Access Highly Accessed Research article

In-vitro evaluation of selected Egyptian traditional herbal medicines for treatment of alzheimer disease

Shereen K Ali1, Ahmed R Hamed12, Maha M Soltan12, Usama M Hegazy3, Esameldin E Elgorashi4, Ibrahim A El-Garf5 and Ahmed A Hussein6*

Author Affiliations

1 Department of Phytochemistry, National Research Centre, 13211 Dokki, Cairo, Egypt

2 Pharmaceutical Research Group, Centre of Excellence for Advanced Sciences, National Research Centre, 13211 Dokki, Cairo, Egypt

3 Molecular Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, 13211 Dokki, Cairo, Egypt

4 Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort 0110, Pretoria, South Africa

5 Department of Botany, Faculty of Science, Cairo University, El-Giza, Egypt

6 Chemistry Department, University of Western Cape, Private Bag X17, Belleville 7535, South Africa

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BMC Complementary and Alternative Medicine 2013, 13:121  doi:10.1186/1472-6882-13-121

Published: 30 May 2013

Abstract

Background

Egyptians recognized the healing power of herbs and used them in their medicinal formulations. Nowadays, “Attarin” drug shops and the public use mainly the Unani medicinal system for treatment of their health problems including improvement of memory and old age related diseases. Numerous medicinal plants have been described in old literature of Arabic traditional medicine for treatment of Alzheimer’s disease (AD) (or to strengthen memory).

Methods

In this study, some of these plants were evaluated against three different preliminary bioassays related to AD to explore the possible way of their bio-interaction. Twenty three selected plants were extracted with methanol and screened in vitro against acetylcholinesterase (AChE) and cycloxygenase-1 (COX-1) enzymes. In addition, anti-oxidant activity using DPPH was determined.

Results

Of the tested plant extracts; Adhatoda vasica and Peganum harmala showed inhibitory effect on AChE at IC50 294 μg/ml and 68 μg/ml respectively. Moreover, A. vasica interacted reversibly with the enzyme while P. harmala showed irreversible inhibition. Ferula assafoetida (IC50 3.2 μg/ml), Syzygium aromaticum (34.9 μg/ml) and Zingiber officinalis (33.6 μg/ml) showed activity against COX-1 enzyme. Potent radical scavenging activity was demonstrated by three plant extracts Terminalia chebula (EC50 2.2 μg/ml), T. arjuna (3.1 μg/ml) and Emblica officinalis (6.3 μg/ml).

Conclusion

Interestingly, differential results have been obtained which indicate the variability of the mode of actions for the selected plants. Additionally, the reversible interaction of A. vasica against AChE and the potent activity of F. assafoetida against COX-1 make them effective, new and promising agents for treatment of AD in the future, either as total extracts or their single bioactive constituents.

Keywords:
Egyptian herbal medicine; Unani medicine; Alzheimer’s disease; Anti-acetylcholinesterase; Anti-inflammatory; Anti-oxidant; Adhatoda vasica; Ferula assafoetida