Changes in CD4+ T-cell markers and association with level of supplemented bacterial in stool. Surface expression of HLA-DR, CD38 and PD-1 were determined on peripheral blood lymphocytes using flow cytometry. These markers were quantified on CD4+ and CD8+ cells globally, and also using Boolean analysis (see Methods and Table 2) (A) Change in percent T-cells CD4 + CD38-HLADR + PD1-, (B) Change in number of CD38 antibodies bound per cell on CD8+ T-cells, (C) Within the Synbiotic group, correlation of the changes in CD38 expression on CD4+ T-cells with the change in fecal P. pentosaceus. Horizontal bars denote the mean. r, Spearman r value.
Schunter et al. BMC Complementary and Alternative Medicine 2012 12:84 doi:10.1186/1472-6882-12-84