Figure 4.

Flow cytometry gating pathway for T-cell activation markers. Initial gating was on lymphocytes as approximated by scatter, then doublets were eliminated. Next, live CD3+ cells were selected, CD14+ cells left behind, then CD4+ and CD8+ cells were gated. For cells in each of the CD4+ and CD8+ gates, individual gates for HLA-DR, CD38, and PD-1 were then established. The frequencies of these populations were analyzed as-is, and also after creation of Boolean combinations of these three groups.