Screening of compounds that up-regulate Foxp3 expression. HEK 293 T cells were transfected with Foxp3 expression plasmids. ( A) Nuclear expression of Foxp3 in HEK 293 T cells was examined by immunofluorescence microscopy. Scale bar, 10 μm. ( B) Foxp3 mRNA expression in transfected HEK 293 T cells was determined by RT-PCR. ( C) Twenty-four hours after transfection, cells were then treated with different compounds for another 24 h. Foxp3 mRNA expression was analyzed by real-time RT-PCR. Data are expressed as mean ± SD, and compared with vector control (expression level of vector control was set as 1.0). Shown are results from three independent experiments.
Yang et al. BMC Complementary and Alternative Medicine 2012 12:64 doi:10.1186/1472-6882-12-64