Open Access Research article

In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell

Hueh Zan Chong12*, Asmah Rahmat1, Swee Keong Yeap3, Abdah Md Akim4, Noorjahan Banu Alitheen3, Fauziah Othman4 and Cheng Lian Gwendoline-Ee5

Author Affiliations

1 Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

2 School of Pharmacy and Health Sciences, International Medical University, No. 126, Jalan Jalil Perkasa 19, Bukit Jalil Kuala Lumpur, 57000 Malaysia

3 Department of Cell and Molecule Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

4 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

5 Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia

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BMC Complementary and Alternative Medicine 2012, 12:35  doi:10.1186/1472-6882-12-35

Published: 4 April 2012

Abstract

Background

Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear.

Results

Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC50 of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study.

Conclusion

Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.

Keywords:
Strobilanthes crispus; MCF-7; Apoptosis; p53