Principal component analysis comparing the influence of EGCG on 67LR versus the control gene. a) For the control gene, the impact of the drug treatment was dependent on whether a gene knockdown was applied (I) or not (II). b) For the 67LR gene, siRNA-induced downregulation altered the extent of its synergy with EGCG (I) and a new data cluster was separated from the samples solely treated with the 67lr-KD (II). When no knockdown was applied, EGCG did not affect the gene regulation (III) in accordance with Figure 3a. (Color code: black labels indicate samples containing EGCG, grey labels indicate no EGCG; Legend: EGCG = EGCG treatment, MEDIA = media control, NV = non-targeting virus, TV = targeting virus, 67lr-KD = 67LR knockdown, control-KD = control knockdown).
Müller and Pfaffl BMC Complementary and Alternative Medicine 2012 12:258 doi:10.1186/1472-6882-12-258