Effects of SCTE on the recruitment of inflammatory cells to bronchoalveolar lavage fluid (BALF) of mice 48 h after the final ovalbumin (OVA) challenge. Cells were isolated by centrifugation and stained with Diff-Quik® Stain reagent. Cell numbers were determined by counting within at least five squares of a hemocytometer using a light microscope. Dead cells, stained with Trypan blue, were excluded from the total cell count. PBS/PBS, PBS-sensitized/challenged, negative control (NC, PBS only administration); OVA/PBS, OVA-sensitized/challenged mice (PBS only administration); OVA/mon, OVA-sensitized/challenged mice (montelukast 30 mg/kg); OVA/SCTE-200, OVA-sensitized/challenged mice (SCTE 200 mg/kg); OVA/SCTE-400, OVA-sensitized/challenged mice (SCTE 400 mg/kg). SCTE or montelukast was given 1 h before the challenge. Significantly different from PBS/PBS, ##P < 0.01; significantly different from OVA, *P < 0.05, **P < 0.01.
Lee et al. BMC Complementary and Alternative Medicine 2012 12:257 doi:10.1186/1472-6882-12-257