Frankincense essential oil-regulated signaling and cell cycle-related proteins expression in pancreatic cancer cells. Human pancreatic cancer cells (5 × 105) were plated into each of 60 mm tissue culture plates for adherence. Cells were treated with either Fractions III (1:600 dilution) or IV (1:1,200 dilution) essential oils. Total cellular proteins were isolated from these cells between 0 (untreated control) and 4 h following essential oil treatment. Levels of Akt and Erk1/2 proteins phosphorylation as well as total cyclin D1 and cdk4 proteins expression were determined using Western blot analysis, and compared between cells with and without frankincense essential oils treatment. Expression of β-actin was also assessed in parallel and used as a protein loading control.
Ni et al. BMC Complementary and Alternative Medicine 2012 12:253 doi:10.1186/1472-6882-12-253