Open Access Open Badges Research article

Young coconut juice can accelerate the healing process of cutaneous wounds

Nisaudah Radenahmad1*, Farid Saleh2*, Ibrahim Sayoh3, Kitja Sawangjaroen4, Patchara Subhadhirasakul1, Piyakorn Boonyoung1, Wilart Rundorn4 and Winyou Mitranun5

Author affiliations

1 Department of Anatomy, Prince of Songkla University, Hat Yai, 90112, Thailand

2 Department of Anatomy, College of Medicine, Al-Imam Muhammad Ibn Saud Islamic University, Riyadh, 13317-4233, Kingdom of Saudi Arabia

3 Faculty of Science and Technology, Princess of Naradhiwas University, Narathiwat, 96000, Thailand

4 Department of Pharmacology, Faculty of Science, Prince of Songkla University, Hat Yai, 90112, Thailand

5 Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, 90112, Thailand

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Citation and License

BMC Complementary and Alternative Medicine 2012, 12:252  doi:10.1186/1472-6882-12-252

Published: 12 December 2012



Estrogen has been reported to accelerate cutaneous wound healing. This research studies the effect of young coconut juice (YCJ), presumably containing estrogen-like substances, on cutaneous wound healing in ovairectomized rats.


Four groups of female rats (6 in each group) were included in this study. These included sham-operated, ovariectomized (ovx), ovx receiving estradiol benzoate (EB) injections intraperitoneally, and ovx receiving YCJ orally. Two equidistant 1-cm full-thickness skin incisional wounds were made two weeks after ovariectomy. The rats were sacrificed at the end of the third and the fourth week of the study, and their serum estradiol (E2) level was measured by chemiluminescent immunoassay. The skin was excised and examined in histological sections stained with H&E, and immunostained using anti-estrogen receptor (ER-α an ER-β) antibodies.


Wound healing was accelerated in ovx rats receiving YCJ, as compared to controls. This was associated with significantly higher density of immunostaining for ER-α an ER-β in keratinocytes, fibroblasts, white blood cells, fat cells, sebaceous gland, skeletal muscles, and hair shafts and follicles. This was also associated with thicker epidermis and dermis, but with thinner hypodermis. In addition, the number and size of immunoreactive hair follicles for both ER-α and ER-β were the highest in the ovx+YCJ group, as compared to the ovx+EB group.


This study demonstrates that YCJ has estrogen-like characteristics, which in turn seem to have beneficial effects on cutaneous wound healing.

Young coconut juice; Wound healing; Estrogen; ER-α; ER-β; Ovariectomy