Optimisation experiment of MTT assay on COS-7 cells. Cells were treated with various concentrations of C. longa hot water extract as indicated in quadruplicate. After 3 days (A); 5 days (B); and 7 days (C) of incubation, cells were subjected to viability analysis using MTT assay with different amounts of MTT (2-8 μg). Bar graph represents a mean value whereas an error bar indicates the uncertainty value of three independent experiments.
Waiyaput et al. BMC Complementary and Alternative Medicine 2012 12:246 doi:10.1186/1472-6882-12-246