Email updates

Keep up to date with the latest news and content from BMC Complementary and Alternative Medicine and BioMed Central.

Open Access Research article

Antimicrobial, antimycobacterial and antibiofilm properties of Couroupita guianensis Aubl. fruit extract

Naif Abdullah Al-Dhabi1*, Chandrasekar Balachandran2, Michael Karunai Raj23, Veeramuthu Duraipandiyan1, Chinnasamy Muthukumar1, Savarimuthu Ignacimuthu2, Inshad Ali Khan4 and Vikrant Singh Rajput4

Author Affiliations

1 Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

2 Division of Microbiology, Entomology Research Institute, Loyola College, Chennai, 600 034, India

3 Research and Development Centre, Orchid Chemicals and Pharmaceuticals Ltd, Sozhanganallur, Chennai, 600119, India

4 Clinical Microbiology Division, Indian Institute of Integrative Medicine, Jammu, 180 001, India

For all author emails, please log on.

BMC Complementary and Alternative Medicine 2012, 12:242  doi:10.1186/1472-6882-12-242

Published: 4 December 2012

Abstract

Background

Couroupita guianensis Aubl. (Lecythidaceae) is commonly called Ayahuma and the Cannonball tree. It is distributed in the tropical regions of northern South America and Southern Caribbean. It has several medicinal properties. It is used to treat hypertension, tumours, pain, inflammatory processes, cold, stomach ache, skin diseases, malaria, wounds and toothache.

Methods

The fruits of Couroupita guianensis were extracted with chloroform. Antimicrobial, antimycobacterial and antibiofilm forming activities of the chloroform extract were investigated. Quantitative estimation of Indirubin, one of the major constituent, was identified by HPLC.

Results

Chloroform extract showed good antimicrobial and antibiofilm forming activities; however it showed low antimycobacterial activity. The zones of inhibition by chloroform extract ranged from 0 to 26 mm. Chloroform extract showed effective antibiofilm activity against Pseudomonas aeruginosa starting from 2 mg/mL BIC, with 52% inhibition of biofilm formation. When the chloroform extract was subjected to HPLC-DAD analysis, along with Indirubin standard, in the same chromatographic conditions, it was found that Indirubin was one of the major compounds in this plant (0.0918% dry weight basis).

Conclusions

The chloroform extract showed good antimicrobial and antibiofilm properties. Chloroform extract can be evaluated further in drug development programmes.

Keywords:
Antimicrobial; Antimycobacterial; Antibiofilm; Couroupita guianensis; Indirubin