Table 1

DNA migration in the comet assay from V79 cells treated with various doses of HK and/or DXR and their respective controls
Treatments(μM) Class* Score* Reduction %;
0 1 2 3
Control 84.3 ± 9.0 10.0 ± 4.0 2.3 ± 2.0 1.6 ± 2.0 20.3 ± 13.0 -
MeOH 87.0 ± 7.0 11.0 ± 5.5 2.3± 1.5 0 18.0 ± 5.1 -
0.5 81.6 ± 4.9 12.3± 3.5 4.3 ± 3.7 5.0 ± 2.8 26.0 ± 13.2 -
1.0 87.0 ± 5.1 11.0 ± 5.2 1.3 ± 0.5 0 13.0 ± 5.0 -
2.0 83.0 ± 3.7 14.0 ± 2.5 2.0 ± 2.0 0 18.0 ± 5.0 -
32 89.0 ± 2.0 10.0 ± 2.0 0.3 ± 0.5 0.6 ± 1.1 9.6 ± 2.3 -
64 88.0 ± 3.6 11.0 ± 4.0 0.6 ± 1.0 1.0 ± 1.0 13.0 ± 3.4 -
128 89.0 ± 1.5 10.0 ± 1.5 1.0 ± 0.0 0 12.0 ± 1.5 -
DXR 41.0 ± 12.0 36.0 ± 12.7 18.0 ± 2.0 4.0 ± 2.5 86.0 ± 11.5a -
DXR + MeOH 46.3 ± 6.8 30.0 ± 6.0 16.3 ± 5.6 6.6 ± 3.0 83.3 ± 14.5a -
0.5 + DXR 73.0 ± 7.0 20.0 ± 7.5 4.6 ± 1.5 2.0 ± 2.0 36.0 ± 6.1a,b 76.0
1.0 + DXR 68.0 ± 2.0 20.6 ± 2.5 8.0 ± 2.0 3.3 ± 0.5 46.0 ± 3.0a,b 60.8
2.0 + DXR 65.0 ± 6.8 27.0 ± 6.6 4.3 ± 2.3 2.6 ± 1.6 44.3 ± 9.2a,b 63.4
32 + DXR 35.0 ± 5.0 33.0 ± 3.0 30.0 ± 4.1b 1.0 ± 1.0 97.0 ± 10.0a -
64 + DXR 32.0 ± 6.1 39.0 ± 3.7 26.0 ± 2.0 2.0 ± 2.0 97.0 ± 10.0a -
128 + DXR 28.0 ± 6.6 40.0 ± 1.0 29.0 ± 5.2b 3.0 ± 2.5 106.0 ± 16.0a -

HK = (−)-hinokinin; MeOH= methanol (0.1%;); DXR= doxorubicin (0.3 μM).

The different doses (0.5, 1.0, 2.0, 32, 64 and 128 μM) correspond to HK.

*Values are the means ± standard deviation.

a Significantly different from control (P<0.05).

b Significantly different from the DXR group (P<0.05).

Resende et al.

Resende et al. BMC Complementary and Alternative Medicine 2012 12:203   doi:10.1186/1472-6882-12-203

Open Data